Marisa Masumi Beppu

Universidade Estadual de Campinas – University of Campinas (UNICAMP)

3D biopolymer-based sensors for Circulating Tumor Cell (CTCs) detection: challenges for viability

Biography

Marisa Beppu is full professor on Chemical Engineering Dept. at the State University of Campinas. She has experience in Chemical Engineering, with an emphasis on working with natural polymers, working mainly on the following topics: thermodynamics of macromolecules, natural polymers (chitosan, silk fibroin and glucomannan), adsorption and separation processes, biomaterials, calcification and phosphates. She has been working on bilateral projects with the USA (MIT), Canada (ULaval), France (EMAlès and UCP), Portugal (INEB and FEUP) and Argentina (UNR). She was the head of the Thermofluiddynamics Department (2004-2006), associate director (2010-2014) and director (2014-2017) of the Faculty of Chemical Engineering,
at Unicamp. Se actuates as Vice-President of University Development (PRDU) at Unicamp (2017-2019), Teaching Representative on the University Council of Unicamp (2019-2021 and 2021-2023), Representative of CRUESP on the Board of Directors of SPPREV (2020-2022). She is a 1D productivity fellow from CNPq and a member of the CNPq Advisory Committee (CA-EQ, 2021-2024).

Abstract

Cancer is the second leading cause of death worldwide, and its early detection, associated with an appropriate therapeutic regimen, is the best option to substantially reduce the risk of mortality [1]. Despite advances in the study of biomarkers for early diagnosis, strategies for their detection still require improvements. One approach for detection of cancer is by capturing circulating tumor cells (CTCs) but both selectivity and sensitivity have been bottlenecks for viability of the detection method [1,2]. Our group has been working on the development of a platform for the diagnosis of oncological diseases from the detection of CTCs by using thin LbL (layer-by-layer) coatings on 3D sensors [2,3]. We explored microfabrication techniques, synthesis of recombinant proteins and layer-by-layer surface functionalization for the development of three-dimensional electrodes aiming detection of different prostate tumor cell lines. To date, we have investigated the impact of 3D substrates and optimized biopolymer LbL coatings [2,3], examining the influence of biopolymer molar masses and electrode geometries on cell adhesion dynamics.

References

  1. Shen, H.; Yang, J.; Chen, Z.; Chen, X.; Wang, L.; Hu, J.; Ji, F.; Xie, G.; Feng, W. A Novel Label-Free and Reusable Electrochemical Cytosensor for Highly Sensitive Detection and Specific Collection of CTCs. Biosens Bioelectron 2016, 81, 495–502, doi:10.1016/j.bios.2016.03.048.
  2. Rocha Neto, J.B.M.; Copes, F.; Chevallier, P.; Vieira, R.S.; da Silva, J.V.L.; Mantovani, D.; Beppu, M.M. Polysaccharide-Based Layer-by-Layer Nanoarchitectonics with Sulfated Chitosan for Tuning Anti-Thrombogenic Properties. Colloids Surf B Biointerfaces 2022, 213, 112359, doi:10.1016/j.colsurfb.2022.112359.
  3. Lima, G.G.; Rocha Neto, J.B.M.; Carvalho, H.F. de; Beppu, M.M. Control of Surface Properties of Hyaluronan/Chitosan Multilayered Coatings for Tumor Cell Capture. Polysaccharides 2021, 2, 387–399, doi:10.3390/polysaccharides2020025.
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